Abstract
High performance liquid chromatography (HPLC) was proven to be useful for the analysis of corticoid (CS) extracted from plasma, urine or adrenal tissue.
In the suitable condition of HPLC (LC-2 type) with Zorbax SIL or CN column, organic solvent (cyclohexane: dichloromethane: ethanol=9: 4: 1 or 50% methanol), and UV 254 nm detector, CS fractions both of polar (6β-OHF, ER, epiER etc.) and less polar group (progesterone, 11-desoxycorticosterone etc.) were separated quantitatively in extracts from plasma and urine. The ratio of each CS-fractions on chromatogram was varied with age and/or diseases of patients.
The serial changes of HPL-chromatogram of CS and its metabolites. in plasma were analyzed after oral administration of prednisolone, betamethasone or triamcinolone and their plasma concentrations contrasted with those of ACTH and endogenous F (cortisol).
