Abstract
Chemiluminescence generated with the reaction of bis (2, 4, 6,-trichlorophenyl) oxalate and hydrogen peroxide was applied to a detection system for HPLC to determine fluorescamine-labeled catecholamines. The sensitivity of the chemiluminescence detection system was 25fmol for fluorescamine-labeled dopamine. Norepinephrine and dopamine in human urine were determined by the use of the new HPLC detection system with the coefficient of variation of less than 4.0%. Good correlations were obtained between the values for both norepinephrine and dopamine by the present method and the convetional fluorescence method.
In order to extend the utility of the new method we are tryin g to apply it to the determination of plasma catecholamines. However, for the determination of them in plasma, more sensitivity is required. For the purpose, we replaced the photomultiplier to a more sensitive one, changed the shape of a flow cell to a spiral type and re-investigated chemiluminescence reaction conditions from the view point of time courses of the reaction.
