METHOD OF DETERMINATION OF IRON IN THE FORM OF FERRIOXAMINE IN URINE

Abstract

A new method was introduced for the determination of iron in the form of ferrioxamine excreted in urine after the injection of desferrioxamine to the patient having iron overload.
Sodium hydroxide solution was added to dissociate iron from ferrioxamine, and iron was co-precipitated with magnesium carbonate powder. Iron in the precipitate was dissolved in hydrochloric acid and determined colorimetrically using thioglycolic acid and orthophenanthroline.
This method does not require a respective control sample as the method of direct colorization of iron in urine, or time consuming procedures as the method of ashing. Unlike the method of ashing and atomic absorption, the contamination of hemoglobin iron does not occur with this method.