Abstract
A simple and rapid procedure has been developed for the determination of serum Non Esterified Fatty Acid (NEFA). This is an extremely sensitive and reproducible method.
We have attempted to reduce the well known interferences by other constituents in body fluid by using a modified extraction solvent and a copper salt solution. In other words, we have successfully reduced the extraction of phospholipids from samples by diluting the CHCI3 extraction reagent with n-Heptane and almost completely reduce the interference of bilirubin with setting the pH range to 8.2-8.3 of the reagent.
We have used 2-(2-Thiazolylazo) p-cresol (TAC, Max. 610nm) as a color reagent. The intensity of color with this reagent was two times higher than an ordinary Cu color reagent such as Bathocuproine, Sodium Dithiocarbamate, or Diethyldithiocarbamate.
