Abstract
A simple and sensitive enzyme-immunoassay technique for quantitation of human α1-microglobulin was established for the first time.The sandwich method, using anti-α1-microglobulin goat immunogiobulin G (lgG)-coated polystyrene beads and horseradish peroxidase-labeled antibody, was employed for immunoassay.Small polystyrene beads were loaded with goat antibody lgG using a simple adsorption technique.This method is highly sensitvie and is capable of detecting minimum concentration of α1-microglobulin in body fluids of 0.5ng/ml.The recovery rates of α1-microglobulin in sera and urine were 97.8% and 98.9%, respectively, and α1-microglobulin determinations on serial dilutions of both samples showed a satisfactory linearity.The coefficierlts of within-assay variation ranged between 2.8-6.2%in sera and 2.3-7.9%in urine, and those of between-assay variation 1.8-4.9%in sera and 1.3-6.0%in urine.α1-microglobulin leveis in the sera of 60normal adults quantitated in this study were found to be 8.82±2.9mg/l (mean±1SD), ranging from 5.89 to 11-75mg/l, While those in random Urines were 1.78±0.90mg/l, ranging from 0.69 to 3.92mg/l.This enzyme-immunoassay method should prove useful determining levels of α1-microglobulin in body fluids in which it might be present only in extremely low levels, and, moreover, is suitable for routine use in clinical laboratories.
