Abstract
simple and rapid methods for the determination of zinc and citric acid in human seminal plasma were examined, using atomic absorption spectrophotometer and isotachophoretic analyzer. Seminal fluid was thoroughly liquefied by standing for 1hr. at room temperature with occasional shaking, follwed by centrifugation (4000rpm). The upper layer (seminal plasma) was diluted 500 times with distilled water and the solution was submitted to atomic absorption spectrophotometer for the measurement of zinc. Citric acid was also measured by isotachophoretic analyzer after 100 times dilution. Those methods were applied to healthy human seminal plasma and the results showed that concentrations of zinc and citric acid were 2.71±0.53mM (n=9) and 37.78±6.07mM (n=9) respectively, and the correlation coefficient (r) between zinc concentration and citric acid was 0.955.
