Abstract
lt was revealed that addition of AC-17 into human serum resulted in falsely elevated bitirubin values when determined as intensity of absorption at 600nm by routine laboratory procedure. As the cause of this false elevation in bi-irubirl value, the formation of chelate of AC-17 with heavy metals (Fe, Cu, etc.) existing as trace elements in the serum may be most important. Coincidentally, no false elevation in serum bilirubin value was recorded in same samples with AC-17 by means of the blank method using blank reagent containing large amount of cystein. This may be related to reduction of chelate compound by cystein.On the other hand, exact measurement of bilirubin in serum samples with AC-17 was achieved by a double wave length assay. In this assay, the difference in the intensity of absorption at 600nm and 520nm was constarlt irrespective of the amount of AC-17in serum samples.
