Solid Phase Immunoassay of Corticosterone Using Fluorescent Tag Labelled Second Antibody

Abstract

Solid phase immunoassay of corticosterone was established using fluorescent tag labelled 2nd antibody and corticosterone conjugated polyacrylamide beads (lmmunobead®).The sensitivity of this assay was 40pg/tube.Light scattering from the beads was negligible in this assay conditbns.The most suitable protein to protect non-specific binding of materials to the assay tubes was highly purified gelatin because of its lower intensity of the back ground signal.