A New Method of Serum Mucoprotein (Acid Soluble Glycoproteins: ASP) Assay Using Coomassie Brilliant Blue G-250

Abstract

A new and simple method for protein assay in strongly acidic solution was developed and applied to the assay of perchloric acid soluble glycoproteins (ASP) in serum. Serum ASP levels were determined directly with the supernatants deproteinized by 0.6M perchloric acid using Coomassie Brilliant Blue G-250. This method required neither pH neutralization nor further precipitation of ASP by phosphotungstic acid.
The comparative studies of ASP and seromucoid fraction have shown that the composi tion of both protein fractions was almost identical. Also the serum levels of ASP was highly correlated with seromucoid levels by Winzler method. The results suggest that ASP determi nation by C.B.B.-G-250 method is thought to be identical with serum mucoprotein assay.