1982 Volume 11 Issue 3 Pages 231-236
A reverse phase high performance liquid chromatography (HPLC) method was developed for the determination of Hypoxanthine, Xanthine, Uric acid, Allopurinol (4-hydroxypyrazolo-(3, 4-d) pyrimidine) and Oxypurinol (4, 6-dihydroxypyrazolo-(3, 4-d) pyrimidine) in human serum and urine.
This technique is as follows: Speciments were deproteinized with Amicon centriflo membrane cone type CF25 (Amicon Corp.) and were injected into the HPLC column directly. 0.04mol/l phosphate buffer, pH 2.2, contained 2% methanol (V/V) was used as the mobilphase and the eluent were monitored at 254nm.
Proposed method, which has good sensitivity (the lower limites of determination 1.0 μmol/l for the compounds), precision (CV, 2-3% for 100 μmol/l compounds) and accuracy (almost 100% recovery), were observed.
This method should be useful in the clinical studies for abnormal purine metabolism.
