The Enzymatic Determination of Lipid Hydroperoxides in Serum

Abstract

A specific determination method of lipid hydroperoxides by the enzymatic reaction using a peroxygenase from pea seads is proposed.
The standard calibration curve showed high linearity in both calorimetry and fluorometry. A linearity was proven up to 3μ moles of linoleic hydroperoxide in the presence of 6p moles of in dole in the calorimetry. Since the sensitivity of the fluorometry using N-methyl in dole was better than that of the calorimetry, the former might be favorable for the clinical application.
The minimum detecting sensitivity was 50 n moles/ml as linoleic hydroperoxide in the fluorometry.
The variaton coefficient ranged within±3.02% and the average percentage recovery was as 101.9% in the fluororometric determination.