Study on Discrepancy between Ultracentrifuge Method and Precipitation Method in Measurement of Plasma HDL Cholesterol

Abstract

It was Observed that the values of HDL-cholesterol (HDL-TC) in plasma by ultracentrifugation method were occationally higher levels than the values of HDL-TC by phosophotungs-MgCl2 (ph.Tg-MgCl2) precipitation method.We studied on the mechanism of this discrepancy between the values of HDL-TC by the two methods.ln the plasma which were shown the discrepancy in values of HDL-TC, sinking pre β band by electropholesis and immunoprecipitation of Lp [a] with HDL fraction were observed.Sinking pre β lipoprotain (Lp. [a]) was separated into HDL fraction by ultracentrifugation.
On the other hand, the supernatant after ph.Tg-MgCl2 precipitation did not contain sinking Pre β lipoprotain (Lp [a]).
These results show that in the plasma which contains sinking pre β lipoprotain, the values of HDL-TC by ultracentrifugation method were higher than the values of HDL-TC by ph.Tg-MgCl2 precipitation method.