Abstract
A highly sensitive fluorescent determination for CPK isoenzyme with continuous flow injection system is described.
In the system, -iquid chromatography with stepwise gradient and stream switching technique with six-port valve are utilized. CPK isoenzyme is separated on a micro column packed with DEAE-sepharose and NADH is produced with a coupled enzymaticreactions.Serum and reagents backgrounds are separated from fluorescence NADH by stream switching technique. A major advantage of the detection system is the sensitive monitoring of the isoenzyme activities.
In the measurement of control serum, coefficient of variation was2.5%for CPK-MM, 6.6%for CPK-MB and 9.3%for CPK-BB (n=10).
We have applied this technique to the patient serum to study about clinical investigation. especially CPK MB and BB activities in the case of myocardial infarction for recent ll months.
