Abstract
Colorimetric methods for the determination of serum guanase, based on the formation of H2O2 by the coupling of xanthine oxidase (XOD) followed by color development with peroxidase-MBTH-aniline derivative system, were deveKoped.
As the aniline derivatives, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-aniline (ALOS) andN-ethyl-N-(3-sulfopropy1)-m-anisidine (ADPS) were used for fixed time assay and continuous monitoring assay, respectiveiy.
The addition of superoxide dismutase (SOD) to the coupling step of XOD markedly improved the linearity of reaction, and the formation of H2O2 was stoichio-metrically measured.
The coefficient variance of normal serum material was less than3.3%both on fixed time and Continuous monitoring assay.
The proposed methods, which were with only two reagents system and relatively higher reproducibility, would be simply applied to commercial automated analyzer and useful for the diagnosis of liver cell damage.
