Application of GC/QMS to Clinical Laboratory Method 1.Analysis of Urinary Steroids

Abstract

A report is presented on the development of a routine GC/MS assay for urinary steroids screening.Urine is enzymatically hydrolyzed with glusulase, and extracted steroids are converted to their corresponding MO-TMS derivatives and quantitated with a quadropole GC/MS system using SIM (Selected lon Monitoring).Up to thirteen steroid smay be simultaneously quantified.
Data from a few of abnormal urine specimens which 17-Ketosteroids total is presented high or low lelvels are reported and compared to healthy urine specimen.
This method is simple, sensitive and specific and is suitable for following the metabolic profiling of steroids in human urine specimens.