1984 Volume 13 Issue 2 Pages 66-73
This paper describes an automated, sensitive method for determination of polyamine in urine.lt is based on luminol-chemiluminescent assaying of hydrogen peroxide generated from polyamine in the presence of putrescine oxidase.
The standard curve obtained with putrescine solutions was linear in the range between 680 pmoles and 20.4 nmoles. The within-assay CV was less than 2%.The results obtained with this method correlated well with those obtained with an enzymatic method based on spectrophotometry of hydrogen peroxide (n=70, y=1.15x-1.77, r=0.994) and with those obtained on high performance liquid chromatography (n=24, y=0.908x+6.41, r=0.995).
The normal range of the polyamine/creatinine ratio in 24 hr urine was less than 34 (μmole/g) for males and less than 50.9 for females. The polyamine/creatinine ratio was elevated remarkably in somepatients with malignant proliferative diseases.
