Measurement of Serum α-Amylase Isozymes Using γ-Cyclodextrin
1984 Volume 13 Issue 2 Pages 93-101
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1984 Volume 13 Issue 2 Pages 93-101
A new method for determining α-amylase isozymes in senum was developed.This method needs the measurement of α-amylase activity in a shgle sample by two different assay methods for the erlzyme.ln one of the two methods, γ-cyclodextrin was used as a substnate.The principle of this differential assay for α-amylase isozymes was based on the fact that γ-cyclodextrin is hydrolyzed by pancreatic α-amylase 3 times mone rapidly than by salivany a-amylase provided that the activities of the two isozymes are of the same value in the other method employing chnomogenic amylose as a substnate.
Analysis of sena from 16 nonmal subjects by the present method showed that pancreatic α-amylase constitutes45.6±10.8% (mean±S.D.) of the total serum α-amylase.This value was in good agreement with those (-50%) reported by others.
