Abstract
A simple high-performance liquid chromatographic method is developed for the fluorimetric determination of forphenicinol in human serum. Forphenicindl is converted to a fluorescent compound by alkaiine ferricyanide oxidation and the compound is then separated within 8 min on a reversed-phase column, Lichrosorb RP-18 with isocratic elution ushg an ammonia buffer (pH 8.5). The fluorescence of the compound is monitored at excitation and emission wavelengths of 385 and 500 nm, respectively.This method permits the sensitve quantification of forphenicinol in serum from men administered forphenichol in clinical investigations.The detection limit of forphenicinol is 1.0 nmol/ml in serum, corresponding to 8 pmol (1.6 ng) in a 100-μl injection volume.
