A New Colorimetric Determination of Atropinesterase Activity Using the 2-Nitrophenyl Hydrazine Coupling Reaction

Abstract

A sensitive and simple method for a color · producing determirlation of tropic acid hydrolyzed from atropine by atropinesterase is described.This method is based on the coupling reaction of carboxylic acid with 2-nitrophenylhydrazine.Tropiohydrazide has a purple color wlth an absorption peak at 535 nm.This method is useful for determing atropinesterase activity in microliter samples or extracts of biopsied tissues.lt can be used also to determine other specific carboxylester hydrolyzing enzyme activities.