Evaluation of CPK-BB Activity in Serum Determined by Stream Switching-System
1984 Volume 13 Issue 5 Pages 258-263
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1984 Volume 13 Issue 5 Pages 258-263
We investigated CPK.BB activity in serum of various individuals at Ueno Civic Hospital.CPK-BB activity was determined by Stream Switching System containing micro colum packed DEAE-sepharose CL 6B, six-port valve, injector and fluorescence detector (Ex 360nm, Em 460nm).The system was useful for the correction of background caused serum and reagents.
The CPK-BB activities of healthy individuals were0.86mU/ml (CPK-BB/total CPK=0.0098) for female (n=35) and 0.92 mU/ml (CPK-BB/total CPK=0.0060) for male (n=35) respectively.We found abnormaiities of CPK-BB activity and relative activity (CPK-BB/total CPK) in patient's serum of renal failure, prostatic cancer, gastric cancer and so on.Especially, in the case of renal failure, CPK-BB activity was determined sensitively without the effects of artifact like vitamines and atbumin in serum.
