Studies on Human α₁-Microglobulin XVl. Establishment of Radioimmunoassay of Double Antibody Method for Human α₁-Micmglobulin and its Clinical Application

Abstract

we have established the double antibody radioimmunoassay for α₁-microgloblin (α₁-m).The range of reproducibilities for three various α₁-m levels was 3.6 to 4.5% for within-assay variation and 4-0 to 8.0% for between-assay variation.
The mean analytical recovery was 94.8% for serum α₁-m and 97.0%for urinary α₁-m, and reliable lirlearities were obtained in dilution tests.Correlation coefficients between the results by RIA and SRID for serum α₁-m and those by RIA and EIA were 0.953 and 0.935, respectively.Using our method.the normal levels of serum α₁-m were 18.8±4-4mg/l (mean±SD) in healthy males and 15.5±3.9mg/l in healthy females.
Measurement of serum α₁-m might be useful as a screening marker for diagnosis in early renal dysfunction and probably as a diagnostic tool for liver failure.