Determination of Polyamines by Radioimmunoassay with Antiserum Against o-Phthalaldehydic Spermine

Abstract

Antisera against spermine were obtained by immunization of rabbits with o-phthalaldehydic spermine conjugated to bovine serum albumin. Characterization of the antiserum and radioimmunoassay of polyamines were described. Polyamine can be determined after reaction with o-phthalaldehyde. As little as 40 and 30 pmol of respective spermine and spermidine were detected by this assay.