Abstract
Mouse monoclonal antibodies against human IgG, IgA and IgM were produced. Monoclonal antibodies were used singly or combinedly. Automated direct turbidimetric procedures, which do not require sample dilution, have been developed for determination of immunoglobulins, IgG, IgA and IgM in serum using a commercial automated chemical analyzer. Three immunoglobulins are measured simultaneously with other routine chemical parameters such as sugars, lipids and enzymes in a single serum sample. Values determined by the new direct automated procedures correlate well with values obtained by single radial immunodiffusion. The within and between assay precisions are lower than 3%. The new method offers great potential for clinical managements of patients.
