Abstract
A simple and sensitive method for the colorimetric determination of hemoglobin in plasma is described. It involves a color reaction with leuco crystal violet, which is oxidized to a colored form by the pseudoperoxidase effect of hemoglobin in the presence of hydrogen peroxide. This assay system is sensitive enough to determine 2mg/dl of hemoglobin and is linear up to 100mg/dl. The precision, that is the coefficient of variation, was 2.75%, and recovery was 99.9%. Close correlation was found between this and the other two methods.
