Abstract
This report describes the serum-dependent nonspecific oxidation of hydrogenperoxide, which strongly interferes with the determination of hydrogen-peroxide by use of continuous flow hydrogen-peroxide electrode.
Properties and characteristics of the H2O2-oxidizing factors in the serum were studied.
Dialysis experiments showed that a nondialyzable, high molecular weight fraction of the pooled serum (named“A” fraction) is mostly responsible for H2O2-oxidation, while a dialyzable, low molecular weight fraction (named“B” fraction) containing various chemical reducing substances such as ascorbic acid, glutathione, cysteine, bilirubin and uric acid is playing only minor role.
The heat-labile and sodium azide-sensitive properties strongly suggests“A” fraction to be a catalase.
