Abstract
Direct radioimmunoassay for the determination of estradiol 3-sulfate 17-glucuronide in serum using highly specific antiserum without prior deconjugation has been developed. Antiserum was raised in the rabbit by immunization with antigen in which the steroid hapten is coupled to a carrier protein through the C-6 position. The proposed method employing this specific antiserum only needs a pretreatment on a Sep-pak C18 cartridge. A simple and reliable assay procedure for the determination of estradiol 3-sulfate 17-glucuronide was established and applied to peripheral blood during pregnancy.
