Abstract
A simple modified spectrophotometric method for determination of arginine aminopeptidase activity in the submaxillary gland of mice was studied. The present method is based on cleaving of the substrate α-N-benzoyle-DL- arginine-ρ-nitroanilide to ρ-nitroaniline which is liberated on enzymatic hydrolysis. Catalization by the enzyme activity is determined by measuring diazotization and coupling converted to a purple azo dye. The intensity of color is measured spectrophotometrically at 546nm. The sensitivity of this method is in accordance with the requirements. Further, it may be useful to determine the activity of arginine aminopeptidase of the submaxillary gland in mice by this method combined with the zymogram method.
