Abstract
The effect of thiamin on excretion of lead into bile was investigeted using isolated rat liver perfusion. The amount of lead excreted into bile was increased by addition of thiamin to the perfusate containing lead acetate. Furthermore, the excreted lead in bile was fractionated by gel chromatography on Sephadex G-75. Lead in the bile was detected in three fractions which corresponded to a high molecular weight and two low molecular weight fractions.
These results lsuggested that the excretion of lead into bile was enhanced by thiamin and that lead taken up in the liver was secreted into bile in which lead might be bound to certain proteins and low molecular substances such as glutathione and thiamin
