Abstract
A high-performance liquid chromatographic method was established for detecting coproporphyrin I and In isomers. Coproporphyrin I and III isomers were separated on a reversed-phase column with isocratic elution and measured by fluorometry. The recovery rate of each coproporphyrin isomer was nearly 100% and the minimum detection limit was less than 1μg/l in urine.
The normal ranges (95% limit) were estimated to be 22.2-57.4μg/g creatinine for coproporphyrin I, 22.1-109.1μg/g creatinine for coproporphyrin III, and 0.71-3.73 for coproporphyrin III/coproporphyrin I ratio.
