Abstract
The authors deviced the assay method for the ratio (Fe2+/Fe3+) of iron in iron binding human serum protein with Nitroso-PSAP and ascorbic acid.
The method consists of (1) elimination of reducing agent (ascorbic acid etc.) from serum protein by the centrifugation with Centriflo membrane cone,(2) liberation of iron (Fe2+, Fe3+) from serum protein by addition of deproteinizing solution cone,(3) determination of Fe2+ with Nitroso-PSAP, and (4) successive determination of Fe2+ formed from Fe3+ by addition of ascorbic acid.
The ratio (Fe2+/Fe3+) of iron bound with protein in sera of 14 healthy subjects at 6 pm was 1.28±0.92 (mean±SD). The ratio in each serum was not changed when the serum samples were stored at 2°C or-13°C for at least 6 days.
