Abstract
A fluorescence high-performance liquid chromatographic (HPLC) method for determinatination of urinary 17-hydroxycorticosteroids (17-OHCS) is described.
Urinary 17-OHCS and 3β, 5α-tetrahydrhydrocortisol (3β, 5α-THF, internal standard) were labelled quantitatively by treatment with dansylhydrazine and the derivatives were clearly separated on Cosmosil 5SL column by use of a mixture of dichloromethane-ethanolwater (88: 5: 7) as a mobile phase. The analytical recovery of added 17-OHCS was almost 100 percent and the reproducibility was also satisfactory. The present method was found to be applicable to urine samples obtained from patients on drug medication such as carbamazepine, which interferes with the determination of urinary 17-OHCS by conventional colo-rimetric methods.
The present method is clinically useful for routine assay of urinary 17-OHCS.
