Fluorimetric Detection of Guanidinosuccinic Acid, Guanidinoacetic Acid, and Creatine Using Post -Column Derivatization with Glucose as a Fluorescence -Producing Reagent

Abstract

A rapid and sensitive high-performance liquid chromatographic method has developed for the analysis of guanidinosuccinic acid, guanidinoacetic acid, and creathe in physiological fluids.
These guanidino compounds are separated on a cation-exchange column and detected with a fiuorimeter which monitors fluorescent derivatives produced by the reaction of the eluted constituents with glucose as a reagent. The fiu Orescent derivatives are measured at 420 nm with excitation at 310nm.
This method was successfully applied to serum and urine in clinical tests.