Abstract
The twin assay of 17α-Hydroxyprogesterone (17-OHP) and human Thyroid Stimulating Hormone (hTSH) in dried blood spotted on filter paper was developed by using Enzyme Linked Immunosorbent Assay (ELISA) method. Horseradish peroxydase (HRP) was used as label enzyme, the enzyme activity of bound fraction after B/F separation was measured by adding hydrogen peroxide (H2O2) and ABTS. The assay range of 17-OHP and hTSH were 2.5-160 ng/ml and 0.5-80μU/ml, respectively. The coefficient of variation for 17-OHP and hTSH were 2.18-7.61% and 3.15-6.83%, respectively. The proposed method was applied to preliminary neonatal screening of congenital adrenal hyperplasia and hypothyroidism using one (3mmφ) dried blood disc.
