Determination of 17-Oxosteroid Sulfates and Glucumnides I. Separation of the Conjugates by Ion Pair Extraction

Abstract

To find a method for separately extracting sulfates and glucuronides conjugates of 17-oxosteroid (17OS) by ion pair extraction, we made 17OS conjugates react with six quarternaryammonium ions at pH 9, 0, and extracted the resulting ion pairs of 17OS sulfates or 17OS glucuronides with quaternaryammonium ions.
As a result, we obtained a method that causes 17OS sulfates and 17OS glucuronides in sample to react with tetrapentylammonium ions (TPA), extracts the produced ion pairs of 17OS sulfates and TPA with benzene, then addes sodium sulfate to the remaining sample, and extracts the ion pairs of 17OS glucuronides and TPA with dichloromethane.