Studies on Generation of Superoxide from Neutrophils in Peripheral Blood and Synovial Fluid of Rheumatoid Arthritis.

Abstract

The superoxide generated from neutrophils in peripheral blood and synovial fluid of rheumatoid arthritis (RA) was measured by flow cytometry and compared with those of healthy controls.
Fluorescence intensity of resting neutrophils in peripheral blood and synovial fluid of RA was significantly higher than that in healthy controls. Phorbol myristate acetate (PMA) stimulated neutrophils from the healthy controls seemed to produce the same degrees of superoxide to those from RA.
The superoxide generation from normal neutrophils was stimulated by synovial fluid of RA.
It suggests that neutrophils of RA were continuously stimulated by same factors in periphral blood and synovial fluid and generate superoxide.