Abstract
We previously noted fibrinogen concentrations were spuriously elevated when determined simultaneously with antithrombin III activity on a Hitachi 705 analyzer. In the current manuscript, we suggest the interference is caused by thrombin-α2 macroglobulin complex in the fibrinogen antiserum (used for immunoturbidimetric fibrinogen assay) that hydrolyzes chromogenic substrate from the antithrombin III activity assay.
