Japanese Journal of Clinical Chemistry
Online ISSN : 2187-4077
Print ISSN : 0370-5633
ISSN-L : 0370-5633
Effect of Phenol on the Activity of Peroxidase Conjugate in the Presence of Sodium Azide
Asako MatuiOsamu SugitaMinoru Yakata
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1990 Volume 19 Issue 1 Pages 67-71

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Abstract
Sodium azide is a well-known bacteriostatic reagent, and is conventionally added to buffer for enzyme immunoassay. Since, however, sodium azide inhibits the peroxidase activity, we cannot use it for enzyme immunoassay with peroxidase conjugate.
In the present study, we found that phenolic group substances of low molecular weight as tyrosine, phenol red, and phenol blocked the interference by sodium azide.
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