Abstract
We developed and tested a simple, practical procedure for the isolation of bilirubin covalently bound to albumin (delta bilirubin: Bd), employing ammonium sulfate precipitation and ultrafiltration. A sample isolated using the procedure had properties consistent with those previously demonstrated for Bd. We were able to isolate 99-100% purified Bd from a sample of serum from jaundiced patients at a recovery rate of 78%.
