1992 Volume 21 Issue 3 Pages 159-165
Anti-bovine serum albumin (BSA) and anti-ovalbumin (OA) antibody concentrations in human sera were determined by the end point method using double antibody solid phase enzyme immunoassay (SP-EIA). The concentration of standard IgG at the end point was measured with a sandwich SP-EIA, and used as the standard for determining antibody concentrations in human sera. Error extents of the sensitivity of double antibody SP-EIA as well as solid phase radioimmunoassay (SP-RIA) showed within a 10-fold range, and its minimum detection levels gave values from 0.10 to 0.92ng/ml using 1% BSA-phosphate buffered saline (PBS) as the blocking reagent, from 1.02 to 9.37ng/ml using 0.8% gelatin-PBS as the blocking reagent. When SP-EIA was applied to determine the human sera antibody, 7 of the 24 samples were judged to be anti-BSA antibody positive samples, and 11 of the 24 samples were judged to be anti-OA antibody positive samples. The positive distribution ranges revealed the values from 41.1 to 370.2ng/ml of anti-BSA antibody concentrations and from 41.1 to 1110.5ng/ml of anti-OA antibody concentrations. However, the titers of anti-BSA and anti-OA antibodies had no significant correlation.
