Abstract
A simple method for the measurement of the catalase activity, which uses an ordinary platinum electrode covered with a dialysis membrane is proposed. The anodic current measured at+0.6 V vs. saturated-calomel electrode (SCE) is proportional to the concentration of hydrogen peroxide in a solution and is decreased by the addition of catalase to the solution. The initial slope of the time-dependent current decrease after the addition of human blood cells to a test solution is directly proportional to the catalase activity. The catalase activity of normal human blood cells was measured as 4.53 mol/hr·ml-1 by this method. This method has an advantage over the previously proposed methods using an oxygen electrode, whereby no deaeration of the test solution is required with the present method.
