A Comparative Study of the Enzymatic Method of Serum Total Cholesterol with Modified Abell-Kendall Method

Abstract

1. In a comparison between modified ALBK (Abell-Levy-Brodie-Kendall) method and the enzymatic method of serum total cholesterol, the enzymatic method had better precision from the results of simultaneous multiplex measurement, duplicate random measurements and differences of results among institutions.
2. The measurement of total cholesterol in SRM 909, that is freeze-dried serum sample, by modified ALBK method and the enzymatic method led to low accuracy in the enzymatic method. While the measurement of total cholesterol in fresh serum led to the same level accuracy between these methods.
3. From these findings, it was considered that the enzymatic method may be used for the standardization of serum total cholesterol measurement, if the intact (no freeze-dried) standard serum is prepared.