1993 Volume 22 Issue 2 Pages 123-132
In order to assess the extent of damage to renal tubular peroxisome granules, we developed a method of measuring the urinary concentration of D-amino acid oxidase, an enzyme well known to be present in these granules. A fundamental study was conducted using this method and its clinical application was also assessed.
The standard curve, reproducibility and recovery rate of the assay were all satisfactory. There was no diurnal variation of urinary excretion or any variation between different ages, and the standard urinary D-amino acid oxidase level was <10.0μg/g creatinine.
Measurement of urinary D-amino acid oxidase levels in diabetic patients and patients with renal damage using this method revealed that this enzyme was a more sensitive marker than urinary microalbumin or other parameters, and it was concluded to be useful for detecting early kidney damage.
