1993 Volume 22 Issue 3 Pages 156-161
Atrial natriuretic peptide (ANP)(10-8mol/l), brain natriuretic peptide (BNP)(10-8mol/l), the protein kinase C (PKC) inhibitors H-7 (10-5mol/l) and staurosporin (10-8mol/l) suppressed aldosterone secretion in cultured bovine adrenal glomerulosa cells by 40 to 50 % compared with untreated control cells. When cells incubated with 10-8mol/l angiotensin II (All) were also treated with ANP, BNP, or PKC inhibitors, aldosterone secretion was 50 to 60 % of that seen in untreated cells. ANP, BNP and PKC inhibitors suppressing the expression of PKC, suggested that PKC plays a role in aldosterone secretion. Our results suggest that ANP and BNP may have an unknown second messenger pathway in which the suppression of PKC is essential for the expression of their physiological actions.
