Immunoradiometric Assay for Group II Phospholipase A2
1993 Volume 22 Issue 3 Pages 180-184
Details
1993 Volume 22 Issue 3 Pages 180-184
An immunoradiometric assay for group II phospholipase A2 (PLA2) in human serum was developed using a combination of two monoclonal antibodies.
Polystyrene beads were coated with monoclonal antibody (No.PL-71), using glutaraldehyde as the coupling reagent, and 125 I-labeled monoclonal antibody (No.PL-78) was prepared using the chloramine T method.
The working range of this assay was 0.5 to 200ng/ml, and the assay was unaffected by the serum matrix. The within-and between-assay coefficients of variation were 1.3-2.9% and 2.6-9.3%, respectively. The group II PLA2 level in sera of healthy individuals was 2.13±0.49ng/ml, while that of patients with rheum atoid arthritis varied between 4.9-111ng/ml. The method described here was highly reliable and required very short incubation time. It appears to be useful in clinical testing for rheumatoid arthritis.
