Recommended Method for Determination of Uric Acid in Serum by HPLC

Abstract

Recommended method for determination of uric acid in serum was established by HPLC.
For deproteinization of 0.2 ml of the test serum, various deproteinizing agents were tested. Among them, 0.3 mol/1 percholric acid was used in this recommended method. After the deproteinization, 0.3 ml of the supernatant was mixed with 0.3 ml of 0.2mol/1 Na2HPO4 solution, and 100μl of the mixture was applied to the ODS column of HPLC. The HPLC determination was completed in 10 min.