Abstract
We developed an anti-GOR assay using the Luminomaster TM system, a fully automated, random access chemiluminescent enzyme immunoassay system. GOR antibody, which is often detected in the serum of patients infected with Hepatitis C virus (HCV), is closely associated with HCV infection.
This new assay provides detection of GOR antibody in 45 min, about half the time of existing EIA methods. The standard curve is 0.1-300 GOR unit/ml (1/10th-300 times cut-off value), givinga detection range about 30 times greater than that of existing assays. This greater detection range allows quantitative determination of low-titer samples without the need for additional detectionprocedures. This assay can also be used to evaluate the clinical efficacy of interferon (IFN) therapy in patients with HCV.
