JSCC Guidelines (1994) on Standardization in Clinical Enzymology

Abstract

Guideline I. JSCC concensus method. This method was established in order to avoid discrepancies between JSCC recommended methods and routine methods in terms of assay temperature. The assay conditions for the JSCC consensus method are those recommended by JSCC, except that incubation is carried out at 37°C. Guideline II. Reagents prepared according to the recommendation of JSCC. These are reagent kits for automated analyzers which are prepared in such a way that the final concentrations of each reagent in the reaction mixture remain the same as those used by the JSCC recommended method, and preservatives or stabilizing agentsadded to the reagents have no influence on enzyme assays. Guideline III. Quality assurance survey. We are hoping that more institutions will report their results according to this method and that the accuracy of enzyme assays will be controlled by the JSCC consensus method.