Abstract
We developed a method to determine rat serum bone specific alkaline phosphatase (ALP) by gel electrophoresis. The method is based on the densitometric scan using polyacrylamide disc gel electrophoresis. The coefficients of variation for bone specific ALP was less than 7% for within-and between-run precisions, respectively, and the detection limit was 10 IU/I. A group of twelve-week-old female rats were ovariectomized and an other group were given sham operations. The bone specific ALP in serum, 6 months after the operation, showed five times higher activity in the ovariectomized groups compared with the sham-operated group. The determination of bone specific ALP will be helpful in animal models of bone disease and the evaluation on the effects of therapeutic agents.
