Abstract
A simple and rapid high-performance liquid chromatographic method for identification and quantitation of several benzodiazepines in human serum is described. Benzodiazepines extracted from serum sample using a solvent extraction were separated on a C18 column and detected at 223 nm. The resultant peak was identified by UV photodiode array detection. Six benzodiazepines were separated within 20 min by an isocratic elution with wateracetonitrile-methanol as a salt-free mobile phase. The detection limits of the benzodiazepines spiked ranged from 8 to 28 ng per ml of serum. The within-day and day-to-day precisions for serum sample gave relative standard deviations of less than 3.1% and 8.6%, respectively.
