Recommended Method for Determination of Tri-glycerides in Serum

Abstract

A reference method for the determination of triglyceride (TG) in serum is described. The method is based on the use of alkaline saponification to hydrolyze triglycerides, followed by an enzymatic determination of the generated glycerol. The principle of the proposed method is as follows. TG is hydrolyzed by alcoholic KOH. Glycerol released is determined by end point analysis, including glycerol kinase, pyruvate kinase, and lactate dehydrogenase reaction. Endogenous glycerol should be subtracted from the results. This method is mainly used for the evaluation of the accuracy of the results obtained by routine methods in the clinical laboratory, determination of assigned value for the routine available commercial reagent kits, and for the control materials of quality assurance, and for the survey materials of quality assessment. Analytical conditions of this method were constituted on the basis of the prior studies done by the old Committee on Reagents in the Kinki Sub-Group of the Analytical Section of JSCC during 1984-89.